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dihydroethidium (dhe) fluorescent probe kit  (Beyotime)


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    Beyotime dihydroethidium (dhe) fluorescent probe kit
    Dihydroethidium (Dhe) Fluorescent Probe Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dihydroethidium+(dhe)+fluorescent+probe+kit/dihydroethidium+dhe/pm39983815-104-8-13
    Average 90 stars, based on 1 article reviews
    dihydroethidium (dhe) fluorescent probe kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Lack of ALCAT1 enhances the protective effects of aerobic exercise on kidney in HFpEF mice.
    Article Snippet: Aims: Heart failure with preserved ejection fraction (HFpEF) is an increasingly prevalent cardiovascular disease, which is often accompanied by kidney dysfunction.. Exercise has been recognized as a feasible strategy to improve renal function.. The aim of this study was to investigate whether aerobic exercise (AE) could ameliorate HFpEF-induced renal injury by regulating the expression of acyl-coenzyme A: lysocardiolipin acyltransferase-1

    Article Title: Electroacupuncture ameliorates spatial learning and memory impairment via attenuating NOX2-related oxidative stress in a rat model of Alzheimer's disease induced by Aβ1-42.
    Article Snippet: ROS levels in the hippocampus were assessed using a ROS Fluorescent Probe-DHE kit (Beyotime, Jiangsu, China) according to the manufacturer’s instructions(23).

    Article Title: Kai-Xin-San Protects Depression Mice Against CORT-Induced Neuronal Injury by Inhibiting Microglia Activation and Oxidative Stress
    Article Snippet: To evaluate the effect of KXS on ROS generation and mitochondrial membrane potential, the fluorescent probe dihydroethidium (DHE) kit (Beyotime, China), CellROX® Oxidative Stress kit (CellROX) and JC-1 Mitochondrial Membrane Potential Assay Kit (Abcam, ab113850) were used to examine the ROS level and alternations of mitochondrial membrane potential in CORT-induced mice or LPS/IFN ɣ -induced BV2 cells following the manufacturer's instructions [ , ].

    Article Title: Aminoguanidine hemisulfate improves mitochondrial autophagy, oxidative stress, and muscle force in Duchenne muscular dystrophy via the AKT/FOXO1 pathway in mdx mice.
    Article Snippet: ROS levels in the TA muscle were evaluated using a 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) kit (S0033S, Beyotime) anda dihydroethidium (DHE) fluorescent probe kit (S0063, Beyotime).

    Article Title: Polysaccharide from Echinacea purpurea plant ameliorates oxidative stress-induced liver injury by promoting Parkin-dependent autophagy.
    Article Snippet: After incubation with dihydroethidine (DHE 10 μM) at 37 ◦C for 30 min, the ROS production was detected with red fluorescence by ROS Fluorescent Probe-DHE (dihydroethidium) assay kit (Beyotime Biotechnology, Jiangsu, China).

    Fluorescence:

    Article Title: Lack of ALCAT1 enhances the protective effects of aerobic exercise on kidney in HFpEF mice.
    Article Snippet: Aims: Heart failure with preserved ejection fraction (HFpEF) is an increasingly prevalent cardiovascular disease, which is often accompanied by kidney dysfunction.. Exercise has been recognized as a feasible strategy to improve renal function.. The aim of this study was to investigate whether aerobic exercise (AE) could ameliorate HFpEF-induced renal injury by regulating the expression of acyl-coenzyme A: lysocardiolipin acyltransferase-1

    Article Title: Electroacupuncture ameliorates spatial learning and memory impairment via attenuating NOX2-related oxidative stress in a rat model of Alzheimer's disease induced by Aβ1-42.
    Article Snippet: ROS levels in the hippocampus were assessed using a ROS Fluorescent Probe-DHE kit (Beyotime, Jiangsu, China) according to the manufacturer’s instructions(23).

    Article Title: Kai-Xin-San Protects Depression Mice Against CORT-Induced Neuronal Injury by Inhibiting Microglia Activation and Oxidative Stress
    Article Snippet: To evaluate the effect of KXS on ROS generation and mitochondrial membrane potential, the fluorescent probe dihydroethidium (DHE) kit (Beyotime, China), CellROX® Oxidative Stress kit (CellROX) and JC-1 Mitochondrial Membrane Potential Assay Kit (Abcam, ab113850) were used to examine the ROS level and alternations of mitochondrial membrane potential in CORT-induced mice or LPS/IFN ɣ -induced BV2 cells following the manufacturer's instructions [ , ].

    Article Title: Aminoguanidine hemisulfate improves mitochondrial autophagy, oxidative stress, and muscle force in Duchenne muscular dystrophy via the AKT/FOXO1 pathway in mdx mice.
    Article Snippet: ROS levels in the TA muscle were evaluated using a 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) kit (S0033S, Beyotime) anda dihydroethidium (DHE) fluorescent probe kit (S0063, Beyotime).

    Article Title: Polysaccharide from Echinacea purpurea plant ameliorates oxidative stress-induced liver injury by promoting Parkin-dependent autophagy.
    Article Snippet: After incubation with dihydroethidine (DHE 10 μM) at 37 ◦C for 30 min, the ROS production was detected with red fluorescence by ROS Fluorescent Probe-DHE (dihydroethidium) assay kit (Beyotime Biotechnology, Jiangsu, China).

    Membrane:

    Article Title: Lack of ALCAT1 enhances the protective effects of aerobic exercise on kidney in HFpEF mice.
    Article Snippet: Aims: Heart failure with preserved ejection fraction (HFpEF) is an increasingly prevalent cardiovascular disease, which is often accompanied by kidney dysfunction.. Exercise has been recognized as a feasible strategy to improve renal function.. The aim of this study was to investigate whether aerobic exercise (AE) could ameliorate HFpEF-induced renal injury by regulating the expression of acyl-coenzyme A: lysocardiolipin acyltransferase-1

    Article Title: Electroacupuncture ameliorates spatial learning and memory impairment via attenuating NOX2-related oxidative stress in a rat model of Alzheimer's disease induced by Aβ1-42.
    Article Snippet: ROS levels in the hippocampus were assessed using a ROS Fluorescent Probe-DHE kit (Beyotime, Jiangsu, China) according to the manufacturer’s instructions(23).

    Article Title: Kai-Xin-San Protects Depression Mice Against CORT-Induced Neuronal Injury by Inhibiting Microglia Activation and Oxidative Stress
    Article Snippet: To evaluate the effect of KXS on ROS generation and mitochondrial membrane potential, the fluorescent probe dihydroethidium (DHE) kit (Beyotime, China), CellROX® Oxidative Stress kit (CellROX) and JC-1 Mitochondrial Membrane Potential Assay Kit (Abcam, ab113850) were used to examine the ROS level and alternations of mitochondrial membrane potential in CORT-induced mice or LPS/IFN ɣ -induced BV2 cells following the manufacturer's instructions [ , ].

    Article Title: Aminoguanidine hemisulfate improves mitochondrial autophagy, oxidative stress, and muscle force in Duchenne muscular dystrophy via the AKT/FOXO1 pathway in mdx mice.
    Article Snippet: ROS levels in the TA muscle were evaluated using a 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) kit (S0033S, Beyotime) anda dihydroethidium (DHE) fluorescent probe kit (S0063, Beyotime).

    Article Title: Polysaccharide from Echinacea purpurea plant ameliorates oxidative stress-induced liver injury by promoting Parkin-dependent autophagy.
    Article Snippet: After incubation with dihydroethidine (DHE 10 μM) at 37 ◦C for 30 min, the ROS production was detected with red fluorescence by ROS Fluorescent Probe-DHE (dihydroethidium) assay kit (Beyotime Biotechnology, Jiangsu, China).

    Staining:

    Article Title: Lack of ALCAT1 enhances the protective effects of aerobic exercise on kidney in HFpEF mice.
    Article Snippet: Aims: Heart failure with preserved ejection fraction (HFpEF) is an increasingly prevalent cardiovascular disease, which is often accompanied by kidney dysfunction.. Exercise has been recognized as a feasible strategy to improve renal function.. The aim of this study was to investigate whether aerobic exercise (AE) could ameliorate HFpEF-induced renal injury by regulating the expression of acyl-coenzyme A: lysocardiolipin acyltransferase-1

    Article Title: Electroacupuncture ameliorates spatial learning and memory impairment via attenuating NOX2-related oxidative stress in a rat model of Alzheimer's disease induced by Aβ1-42.
    Article Snippet: ROS levels in the hippocampus were assessed using a ROS Fluorescent Probe-DHE kit (Beyotime, Jiangsu, China) according to the manufacturer’s instructions(23).

    Article Title: Kai-Xin-San Protects Depression Mice Against CORT-Induced Neuronal Injury by Inhibiting Microglia Activation and Oxidative Stress
    Article Snippet: To evaluate the effect of KXS on ROS generation and mitochondrial membrane potential, the fluorescent probe dihydroethidium (DHE) kit (Beyotime, China), CellROX® Oxidative Stress kit (CellROX) and JC-1 Mitochondrial Membrane Potential Assay Kit (Abcam, ab113850) were used to examine the ROS level and alternations of mitochondrial membrane potential in CORT-induced mice or LPS/IFN ɣ -induced BV2 cells following the manufacturer's instructions [ , ].

    Article Title: Aminoguanidine hemisulfate improves mitochondrial autophagy, oxidative stress, and muscle force in Duchenne muscular dystrophy via the AKT/FOXO1 pathway in mdx mice.
    Article Snippet: ROS levels in the TA muscle were evaluated using a 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) kit (S0033S, Beyotime) anda dihydroethidium (DHE) fluorescent probe kit (S0063, Beyotime).

    Article Title: Polysaccharide from Echinacea purpurea plant ameliorates oxidative stress-induced liver injury by promoting Parkin-dependent autophagy.
    Article Snippet: After incubation with dihydroethidine (DHE 10 μM) at 37 ◦C for 30 min, the ROS production was detected with red fluorescence by ROS Fluorescent Probe-DHE (dihydroethidium) assay kit (Beyotime Biotechnology, Jiangsu, China).

    Concentration Assay:

    Article Title: Lack of ALCAT1 enhances the protective effects of aerobic exercise on kidney in HFpEF mice.
    Article Snippet: Aims: Heart failure with preserved ejection fraction (HFpEF) is an increasingly prevalent cardiovascular disease, which is often accompanied by kidney dysfunction.. Exercise has been recognized as a feasible strategy to improve renal function.. The aim of this study was to investigate whether aerobic exercise (AE) could ameliorate HFpEF-induced renal injury by regulating the expression of acyl-coenzyme A: lysocardiolipin acyltransferase-1

    Article Title: Electroacupuncture ameliorates spatial learning and memory impairment via attenuating NOX2-related oxidative stress in a rat model of Alzheimer's disease induced by Aβ1-42.
    Article Snippet: ROS levels in the hippocampus were assessed using a ROS Fluorescent Probe-DHE kit (Beyotime, Jiangsu, China) according to the manufacturer’s instructions(23).

    Article Title: Kai-Xin-San Protects Depression Mice Against CORT-Induced Neuronal Injury by Inhibiting Microglia Activation and Oxidative Stress
    Article Snippet: To evaluate the effect of KXS on ROS generation and mitochondrial membrane potential, the fluorescent probe dihydroethidium (DHE) kit (Beyotime, China), CellROX® Oxidative Stress kit (CellROX) and JC-1 Mitochondrial Membrane Potential Assay Kit (Abcam, ab113850) were used to examine the ROS level and alternations of mitochondrial membrane potential in CORT-induced mice or LPS/IFN ɣ -induced BV2 cells following the manufacturer's instructions [ , ].

    Article Title: Aminoguanidine hemisulfate improves mitochondrial autophagy, oxidative stress, and muscle force in Duchenne muscular dystrophy via the AKT/FOXO1 pathway in mdx mice.
    Article Snippet: ROS levels in the TA muscle were evaluated using a 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) kit (S0033S, Beyotime) anda dihydroethidium (DHE) fluorescent probe kit (S0063, Beyotime).

    Article Title: Polysaccharide from Echinacea purpurea plant ameliorates oxidative stress-induced liver injury by promoting Parkin-dependent autophagy.
    Article Snippet: After incubation with dihydroethidine (DHE 10 μM) at 37 ◦C for 30 min, the ROS production was detected with red fluorescence by ROS Fluorescent Probe-DHE (dihydroethidium) assay kit (Beyotime Biotechnology, Jiangsu, China).

    Immunohistochemistry:

    Article Title: Lack of ALCAT1 enhances the protective effects of aerobic exercise on kidney in HFpEF mice.
    Article Snippet: Aims: Heart failure with preserved ejection fraction (HFpEF) is an increasingly prevalent cardiovascular disease, which is often accompanied by kidney dysfunction.. Exercise has been recognized as a feasible strategy to improve renal function.. The aim of this study was to investigate whether aerobic exercise (AE) could ameliorate HFpEF-induced renal injury by regulating the expression of acyl-coenzyme A: lysocardiolipin acyltransferase-1

    Article Title: Electroacupuncture ameliorates spatial learning and memory impairment via attenuating NOX2-related oxidative stress in a rat model of Alzheimer's disease induced by Aβ1-42.
    Article Snippet: ROS levels in the hippocampus were assessed using a ROS Fluorescent Probe-DHE kit (Beyotime, Jiangsu, China) according to the manufacturer’s instructions(23).

    Article Title: Kai-Xin-San Protects Depression Mice Against CORT-Induced Neuronal Injury by Inhibiting Microglia Activation and Oxidative Stress
    Article Snippet: To evaluate the effect of KXS on ROS generation and mitochondrial membrane potential, the fluorescent probe dihydroethidium (DHE) kit (Beyotime, China), CellROX® Oxidative Stress kit (CellROX) and JC-1 Mitochondrial Membrane Potential Assay Kit (Abcam, ab113850) were used to examine the ROS level and alternations of mitochondrial membrane potential in CORT-induced mice or LPS/IFN ɣ -induced BV2 cells following the manufacturer's instructions [ , ].

    Article Title: Aminoguanidine hemisulfate improves mitochondrial autophagy, oxidative stress, and muscle force in Duchenne muscular dystrophy via the AKT/FOXO1 pathway in mdx mice.
    Article Snippet: ROS levels in the TA muscle were evaluated using a 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) kit (S0033S, Beyotime) anda dihydroethidium (DHE) fluorescent probe kit (S0063, Beyotime).

    Article Title: Polysaccharide from Echinacea purpurea plant ameliorates oxidative stress-induced liver injury by promoting Parkin-dependent autophagy.
    Article Snippet: After incubation with dihydroethidine (DHE 10 μM) at 37 ◦C for 30 min, the ROS production was detected with red fluorescence by ROS Fluorescent Probe-DHE (dihydroethidium) assay kit (Beyotime Biotechnology, Jiangsu, China).

    Activity Assay:

    Article Title: Lack of ALCAT1 enhances the protective effects of aerobic exercise on kidney in HFpEF mice.
    Article Snippet: Aims: Heart failure with preserved ejection fraction (HFpEF) is an increasingly prevalent cardiovascular disease, which is often accompanied by kidney dysfunction.. Exercise has been recognized as a feasible strategy to improve renal function.. The aim of this study was to investigate whether aerobic exercise (AE) could ameliorate HFpEF-induced renal injury by regulating the expression of acyl-coenzyme A: lysocardiolipin acyltransferase-1

    Article Title: Electroacupuncture ameliorates spatial learning and memory impairment via attenuating NOX2-related oxidative stress in a rat model of Alzheimer's disease induced by Aβ1-42.
    Article Snippet: ROS levels in the hippocampus were assessed using a ROS Fluorescent Probe-DHE kit (Beyotime, Jiangsu, China) according to the manufacturer’s instructions(23).

    Article Title: Kai-Xin-San Protects Depression Mice Against CORT-Induced Neuronal Injury by Inhibiting Microglia Activation and Oxidative Stress
    Article Snippet: To evaluate the effect of KXS on ROS generation and mitochondrial membrane potential, the fluorescent probe dihydroethidium (DHE) kit (Beyotime, China), CellROX® Oxidative Stress kit (CellROX) and JC-1 Mitochondrial Membrane Potential Assay Kit (Abcam, ab113850) were used to examine the ROS level and alternations of mitochondrial membrane potential in CORT-induced mice or LPS/IFN ɣ -induced BV2 cells following the manufacturer's instructions [ , ].

    Article Title: Aminoguanidine hemisulfate improves mitochondrial autophagy, oxidative stress, and muscle force in Duchenne muscular dystrophy via the AKT/FOXO1 pathway in mdx mice.
    Article Snippet: ROS levels in the TA muscle were evaluated using a 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) kit (S0033S, Beyotime) anda dihydroethidium (DHE) fluorescent probe kit (S0063, Beyotime).

    Article Title: Polysaccharide from Echinacea purpurea plant ameliorates oxidative stress-induced liver injury by promoting Parkin-dependent autophagy.
    Article Snippet: After incubation with dihydroethidine (DHE 10 μM) at 37 ◦C for 30 min, the ROS production was detected with red fluorescence by ROS Fluorescent Probe-DHE (dihydroethidium) assay kit (Beyotime Biotechnology, Jiangsu, China).

    Control:

    Article Title: Lack of ALCAT1 enhances the protective effects of aerobic exercise on kidney in HFpEF mice.
    Article Snippet: Aims: Heart failure with preserved ejection fraction (HFpEF) is an increasingly prevalent cardiovascular disease, which is often accompanied by kidney dysfunction.. Exercise has been recognized as a feasible strategy to improve renal function.. The aim of this study was to investigate whether aerobic exercise (AE) could ameliorate HFpEF-induced renal injury by regulating the expression of acyl-coenzyme A: lysocardiolipin acyltransferase-1

    Article Title: Electroacupuncture ameliorates spatial learning and memory impairment via attenuating NOX2-related oxidative stress in a rat model of Alzheimer's disease induced by Aβ1-42.
    Article Snippet: ROS levels in the hippocampus were assessed using a ROS Fluorescent Probe-DHE kit (Beyotime, Jiangsu, China) according to the manufacturer’s instructions(23).

    Article Title: Kai-Xin-San Protects Depression Mice Against CORT-Induced Neuronal Injury by Inhibiting Microglia Activation and Oxidative Stress
    Article Snippet: To evaluate the effect of KXS on ROS generation and mitochondrial membrane potential, the fluorescent probe dihydroethidium (DHE) kit (Beyotime, China), CellROX® Oxidative Stress kit (CellROX) and JC-1 Mitochondrial Membrane Potential Assay Kit (Abcam, ab113850) were used to examine the ROS level and alternations of mitochondrial membrane potential in CORT-induced mice or LPS/IFN ɣ -induced BV2 cells following the manufacturer's instructions [ , ].

    Article Title: Aminoguanidine hemisulfate improves mitochondrial autophagy, oxidative stress, and muscle force in Duchenne muscular dystrophy via the AKT/FOXO1 pathway in mdx mice.
    Article Snippet: ROS levels in the TA muscle were evaluated using a 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) kit (S0033S, Beyotime) anda dihydroethidium (DHE) fluorescent probe kit (S0063, Beyotime).

    Article Title: Polysaccharide from Echinacea purpurea plant ameliorates oxidative stress-induced liver injury by promoting Parkin-dependent autophagy.
    Article Snippet: After incubation with dihydroethidine (DHE 10 μM) at 37 ◦C for 30 min, the ROS production was detected with red fluorescence by ROS Fluorescent Probe-DHE (dihydroethidium) assay kit (Beyotime Biotechnology, Jiangsu, China).

    Expressing:

    Article Title: Lack of ALCAT1 enhances the protective effects of aerobic exercise on kidney in HFpEF mice.
    Article Snippet: Aims: Heart failure with preserved ejection fraction (HFpEF) is an increasingly prevalent cardiovascular disease, which is often accompanied by kidney dysfunction.. Exercise has been recognized as a feasible strategy to improve renal function.. The aim of this study was to investigate whether aerobic exercise (AE) could ameliorate HFpEF-induced renal injury by regulating the expression of acyl-coenzyme A: lysocardiolipin acyltransferase-1

    Article Title: Electroacupuncture ameliorates spatial learning and memory impairment via attenuating NOX2-related oxidative stress in a rat model of Alzheimer's disease induced by Aβ1-42.
    Article Snippet: ROS levels in the hippocampus were assessed using a ROS Fluorescent Probe-DHE kit (Beyotime, Jiangsu, China) according to the manufacturer’s instructions(23).

    Article Title: Kai-Xin-San Protects Depression Mice Against CORT-Induced Neuronal Injury by Inhibiting Microglia Activation and Oxidative Stress
    Article Snippet: To evaluate the effect of KXS on ROS generation and mitochondrial membrane potential, the fluorescent probe dihydroethidium (DHE) kit (Beyotime, China), CellROX® Oxidative Stress kit (CellROX) and JC-1 Mitochondrial Membrane Potential Assay Kit (Abcam, ab113850) were used to examine the ROS level and alternations of mitochondrial membrane potential in CORT-induced mice or LPS/IFN ɣ -induced BV2 cells following the manufacturer's instructions [ , ].

    Article Title: Aminoguanidine hemisulfate improves mitochondrial autophagy, oxidative stress, and muscle force in Duchenne muscular dystrophy via the AKT/FOXO1 pathway in mdx mice.
    Article Snippet: ROS levels in the TA muscle were evaluated using a 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) kit (S0033S, Beyotime) anda dihydroethidium (DHE) fluorescent probe kit (S0063, Beyotime).

    Article Title: Polysaccharide from Echinacea purpurea plant ameliorates oxidative stress-induced liver injury by promoting Parkin-dependent autophagy.
    Article Snippet: After incubation with dihydroethidine (DHE 10 μM) at 37 ◦C for 30 min, the ROS production was detected with red fluorescence by ROS Fluorescent Probe-DHE (dihydroethidium) assay kit (Beyotime Biotechnology, Jiangsu, China).



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    Melatonin protects mitochondrial homeostasis by reducing mitochondrial membrane potential (MMP) and reactive oxygen species <t>(ROS)</t> in nucleus pulposus-derived mesenchymal stem cells (NPMSCs). ( A ) <t>Fluorescence</t> results of ROS in the different groups. Red fluorescence represents a high level of ROS ( B ) Quantitative analysis of ROS fluorescence results. Quantitative analysis of MMP results. ( C ) Fluorescence results of MMP in the different groups. Red fluorescence represents the mitochondrial aggregate JC-1 and green fluorescence indicates the monomeric JC-1 (White scale bar = 100 μm; red scale bar = 25 μm). ( D ) Quantitative analysis of MMP results. All data are the mean ± SD. **: P < 0.01 compared with the control group; ## : P < 0.01 compared with the H 2 O 2 group; && : P < 0.01 compared with the Melatonin group. PI3K-IN-1: PI3K-IN-1 group.
    Ros Detection Fluorescent Probe Dihydroethidium (Dhe) Kit, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dihydroethidium dhe fluorescent probe kit  (Danaher Inc)
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    Melatonin protects mitochondrial homeostasis by reducing mitochondrial membrane potential (MMP) and reactive oxygen species <t>(ROS)</t> in nucleus pulposus-derived mesenchymal stem cells (NPMSCs). ( A ) <t>Fluorescence</t> results of ROS in the different groups. Red fluorescence represents a high level of ROS ( B ) Quantitative analysis of ROS fluorescence results. Quantitative analysis of MMP results. ( C ) Fluorescence results of MMP in the different groups. Red fluorescence represents the mitochondrial aggregate JC-1 and green fluorescence indicates the monomeric JC-1 (White scale bar = 100 μm; red scale bar = 25 μm). ( D ) Quantitative analysis of MMP results. All data are the mean ± SD. **: P < 0.01 compared with the control group; ## : P < 0.01 compared with the H 2 O 2 group; && : P < 0.01 compared with the Melatonin group. PI3K-IN-1: PI3K-IN-1 group.
    Dihydroethidium Dhe Fluorescent Probe Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ros fluorescent probe-dhe (dihydroethidium) assay kit  (Beyotime)
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    Beyotime ros fluorescent probe-dhe (dihydroethidium) assay kit
    Melatonin protects mitochondrial homeostasis by reducing mitochondrial membrane potential (MMP) and reactive oxygen species <t>(ROS)</t> in nucleus pulposus-derived mesenchymal stem cells (NPMSCs). ( A ) <t>Fluorescence</t> results of ROS in the different groups. Red fluorescence represents a high level of ROS ( B ) Quantitative analysis of ROS fluorescence results. Quantitative analysis of MMP results. ( C ) Fluorescence results of MMP in the different groups. Red fluorescence represents the mitochondrial aggregate JC-1 and green fluorescence indicates the monomeric JC-1 (White scale bar = 100 μm; red scale bar = 25 μm). ( D ) Quantitative analysis of MMP results. All data are the mean ± SD. **: P < 0.01 compared with the control group; ## : P < 0.01 compared with the H 2 O 2 group; && : P < 0.01 compared with the Melatonin group. PI3K-IN-1: PI3K-IN-1 group.
    Ros Fluorescent Probe Dhe (Dihydroethidium) Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dihydroethidium+(dhe)+fluorescent+probe+kit/dihydroethidium+dhe/pm35843188-88-22-28
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    Image Search Results


    BMSC-Fe 3 O 4 -SMF-Exos Mitigate Intracellular ROS in Irradiated BMSCs. ( a , c ) Intracellular total ROS levels in BMSCs were evaluated utilizing the 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) fluorescent probe, accompanied by the relevant quantitative determination. ( b , d ) Superoxide anion (O 2 − ) production in irradiated BMSCs was monitored with the DHE fluorescent probe, and concurrent quantitative analysis was implemented. ( e ) The concentration of MDA in irradiated BMSCs was quantified. ( f , g ) The levels of SOD in irradiated BMSCs were measured at the 24 h and 48 h time points, separately. n = 3; (**) p < 0.01; (***) p < 0.001, and (****) p < 0.0001.

    Journal: Bioengineering

    Article Title: Fe 3 O 4 Magnetic Nanoparticles and Static Magnetic Field Stimulated BMSC-Derived Exosomes Promoted Osteogenesis and Alleviated Oxidative Stress in Irradiated BMSCs Through miR-429/NOG Pathway

    doi: 10.3390/bioengineering13040402

    Figure Lengend Snippet: BMSC-Fe 3 O 4 -SMF-Exos Mitigate Intracellular ROS in Irradiated BMSCs. ( a , c ) Intracellular total ROS levels in BMSCs were evaluated utilizing the 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) fluorescent probe, accompanied by the relevant quantitative determination. ( b , d ) Superoxide anion (O 2 − ) production in irradiated BMSCs was monitored with the DHE fluorescent probe, and concurrent quantitative analysis was implemented. ( e ) The concentration of MDA in irradiated BMSCs was quantified. ( f , g ) The levels of SOD in irradiated BMSCs were measured at the 24 h and 48 h time points, separately. n = 3; (**) p < 0.01; (***) p < 0.001, and (****) p < 0.0001.

    Article Snippet: Similarly, intracellular superoxide anion levels were measured using a Dihydroethidium (DHE) Superoxide Anion Fluorescent Probe Kit (G1904-100T, Servicebio, Wuhan, Hubei, China) in full accordance with the manufacturer’s instructions.

    Techniques: Irradiation, Concentration Assay

    Antioxidative strategies, mitochondrial safeguarding, and anti-aging interventions of QOP@P1R16 hydrogel. (A) Optical image of DPPH scavenging test on hydrogels. (B) DPPH scavenging rate of hydrogels. (C–D) ROS flow cytometry with different hydrogel and quantitative analysis results. (E) DCFH-DA probe experiment, (F) mitochondrial membrane potential detection kit, (G) β-galactosidase staining experiment, (H) γ -H2AX immunofluorescence staining and (I) p21immunofluorescence staining on different groups. (J–K) Quantitative analysis results of (E–I). The data are presented as the mean ± SD (n = 3). ∗(p < 0.05), ∗∗(p < 0.01), ∗∗∗(p < 0.001) and ∗∗∗∗ (p < 0.0001) indicate significant differences compared to PBS, &(p < 0.05),&&(p < 0.01), &&&(p < 0.001) and &&&&(p < 0.0001) compared to IR.

    Journal: Materials Today Bio

    Article Title: A multifunctional parathyroid hormone-related supramolecular peptide-loaded dual network hydrogel for radiation-induced wound repair

    doi: 10.1016/j.mtbio.2025.101807

    Figure Lengend Snippet: Antioxidative strategies, mitochondrial safeguarding, and anti-aging interventions of QOP@P1R16 hydrogel. (A) Optical image of DPPH scavenging test on hydrogels. (B) DPPH scavenging rate of hydrogels. (C–D) ROS flow cytometry with different hydrogel and quantitative analysis results. (E) DCFH-DA probe experiment, (F) mitochondrial membrane potential detection kit, (G) β-galactosidase staining experiment, (H) γ -H2AX immunofluorescence staining and (I) p21immunofluorescence staining on different groups. (J–K) Quantitative analysis results of (E–I). The data are presented as the mean ± SD (n = 3). ∗(p < 0.05), ∗∗(p < 0.01), ∗∗∗(p < 0.001) and ∗∗∗∗ (p < 0.0001) indicate significant differences compared to PBS, &(p < 0.05),&&(p < 0.01), &&&(p < 0.001) and &&&&(p < 0.0001) compared to IR.

    Article Snippet: The production of ROS in irradiated L929 cells and the protective effects of the hydrogel were measured using the ROS-detecting fluorescent probe dihydroethidium (DHE) assay kit (KeyGen, China).

    Techniques: Flow Cytometry, Membrane, Staining, Immunofluorescence

    Melatonin protects mitochondrial homeostasis by reducing mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) in nucleus pulposus-derived mesenchymal stem cells (NPMSCs). ( A ) Fluorescence results of ROS in the different groups. Red fluorescence represents a high level of ROS ( B ) Quantitative analysis of ROS fluorescence results. Quantitative analysis of MMP results. ( C ) Fluorescence results of MMP in the different groups. Red fluorescence represents the mitochondrial aggregate JC-1 and green fluorescence indicates the monomeric JC-1 (White scale bar = 100 μm; red scale bar = 25 μm). ( D ) Quantitative analysis of MMP results. All data are the mean ± SD. **: P < 0.01 compared with the control group; ## : P < 0.01 compared with the H 2 O 2 group; && : P < 0.01 compared with the Melatonin group. PI3K-IN-1: PI3K-IN-1 group.

    Journal: Journal of Orthopaedic Translation

    Article Title: Melatonin alleviates oxidative stress-induced injury to nucleus pulposus-derived mesenchymal stem cells through activating PI3K/Akt pathway

    doi: 10.1016/j.jot.2023.10.002

    Figure Lengend Snippet: Melatonin protects mitochondrial homeostasis by reducing mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) in nucleus pulposus-derived mesenchymal stem cells (NPMSCs). ( A ) Fluorescence results of ROS in the different groups. Red fluorescence represents a high level of ROS ( B ) Quantitative analysis of ROS fluorescence results. Quantitative analysis of MMP results. ( C ) Fluorescence results of MMP in the different groups. Red fluorescence represents the mitochondrial aggregate JC-1 and green fluorescence indicates the monomeric JC-1 (White scale bar = 100 μm; red scale bar = 25 μm). ( D ) Quantitative analysis of MMP results. All data are the mean ± SD. **: P < 0.01 compared with the control group; ## : P < 0.01 compared with the H 2 O 2 group; && : P < 0.01 compared with the Melatonin group. PI3K-IN-1: PI3K-IN-1 group.

    Article Snippet: In order to verify the effect of melatonin on ROS production under oxidative stress, ROS fluorescence probe dihydroethidium (DHE) kit (KeyGen, China) was used to detect ROS production in NPMSCs.

    Techniques: Membrane, Derivative Assay, Fluorescence, Control